Product Info Summary
SKU: | M01901-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-gamma Catenin Antibody Picoband™ (monoclonal, 4C12D7)
View all gamma Catenin Antibodies
SKU/Catalog Number
M01901-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-gamma Catenin Antibody Picoband™ (monoclonal, 4C12D7) catalog # M01901-3. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-gamma Catenin Antibody Picoband™ (monoclonal, 4C12D7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01901-3)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
4C12D7
Isotype
Mouse IgG1
Immunogen
E.coli-derived human gamma Catenin recombinant protein (Position: M556-A745). Human gamma Catenin shares 98% amino acid (aa) sequence identity with both mouse and rat gamma Catenin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01901-3 is reactive to JUP in Human, Mouse, Rat
Applications
M01901-3 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
82 kDa
Calculated molecular weight
81.745kDa
Background of gamma Catenin
Junction plakoglobin(JUP), also known as gamma-catenin, is a protein that in humans is encoded by the JUP gene. It is a member of the catenin protein family and homologous to β-catenin, and it is mapped to 17q21.2. This gene encodes a major cytoplasmic protein that is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins. Meanwhile, JUP may have distinct roles in Wnt signaling and cancer via differential effects on downstream target genes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of gamma Catenin using anti-gamma Catenin antibody (M01901-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-gamma Catenin antigen affinity purified monoclonal antibody (Catalog # M01901-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for gamma Catenin at approximately 82 kDa. The expected band size for gamma Catenin is at 82 kDa.
Click image to see more details
Figure 2. IHC analysis of gamma Catenin using anti-gamma Catenin antibody (M01901-3).
gamma Catenin was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-gamma Catenin Antibody (M01901-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of gamma Catenin using anti-gamma Catenin antibody (M01901-3).
gamma Catenin was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-gamma Catenin Antibody (M01901-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of gamma Catenin using anti-gamma Catenin antibody (M01901-3).
gamma Catenin was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-gamma Catenin Antibody (M01901-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of MCF-7 cells using anti-gamma Catenin antibody (M01901-3).
Overlay histogram showing MCF-7 cells stained with M01901-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-gamma Catenin Antibody (M01901-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For JUP (Source: Uniprot.org, NCBI)
Gene Name
JUP
Full Name
Junction plakoglobin
Weight
81.745kDa
Superfamily
beta-catenin family
Alternative Names
catenin (cadherin-associated protein), gamma (80kD); catenin (cadherin-associated protein), gamma 80kDa; Catenin gamma; Desmoplakin III; desmoplakin-3; DP3CTNNG; DPIII; gamma-catenin; junction plakoglobin; PDGBARVD12; PKGB JUP CTNNG, DP3, DPIII, PDGB, PG, PKGB junction plakoglobin junction plakoglobin|catenin (cadherin-associated protein), gamma 80kDa|desmoplakin III|desmoplakin-3|desmosomal protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JUP, check out the JUP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JUP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-gamma Catenin Antibody Picoband™ (monoclonal, 4C12D7) (M01901-3)
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